- ￥3120
- biorbyt
- orb5869
- 英国
- 2025年12月03日
- FC, ICC
- Rabbit
- Human
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- 详细信息
- 技术资料
- 抗体名：
CK10 Rabbit Polyclonal Antibody抗体
- 抗体英文名：
CK10 Rabbit Polyclonal Antibody
- 靶点：
KRT10
- 浓度：
1mg/ml
- 应用范围：
FC, ICC
- 宿主：
Rabbit
- 适应物种：
Human
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb5869
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human CK10 (151-250/584aa)
- 规格：
50 ul
产品描述：CK10 Rabbit Polyclonal Antibody
别名:56 kDa cytokeratin; BCIE; BIE; CK 10; CK-10; ck10; Cytokeratin 10; Cytokeratin 6B; Cytokeratin VIB; Cytokeratin-10; Cytokeratin10; EHK; k10; K1C10_HUMAN; Keratin 10; Keratin; Keratin type i cytoskeletal 10; Keratin type I cytoskeletal 59 kDa; Keratin-10; Keratin10; kpp; Krt 1 10; Krt 10; krt10; type I cytoskeletal 10; Type I keratin Ka10; Keratin, type I cytoskeletal 10; Cytokeratin-10; K10.
免疫原：KLH conjugated synthetic peptide derived from human CK10 (151-250/584aa)
预测反应性:Bovine, Canine, Equine, Guinea pig, Porcine, Rabbit
分子量:56 kDa
应用稀释比例：ICC/IF=1:100-500, Flow-Cyt=1μg/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P13645
Note:For research use only.
别名:56 kDa cytokeratin; BCIE; BIE; CK 10; CK-10; ck10; Cytokeratin 10; Cytokeratin 6B; Cytokeratin VIB; Cytokeratin-10; Cytokeratin10; EHK; k10; K1C10_HUMAN; Keratin 10; Keratin; Keratin type i cytoskeletal 10; Keratin type I cytoskeletal 59 kDa; Keratin-10; Keratin10; kpp; Krt 1 10; Krt 10; krt10; type I cytoskeletal 10; Type I keratin Ka10; Keratin, type I cytoskeletal 10; Cytokeratin-10; K10.
免疫原：KLH conjugated synthetic peptide derived from human CK10 (151-250/584aa)
预测反应性:Bovine, Canine, Equine, Guinea pig, Porcine, Rabbit
分子量:56 kDa
应用稀释比例：ICC/IF=1:100-500, Flow-Cyt=1μg/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P13645
Note:For research use only.
A431 cell, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CK10) polyclonal Antibody, Unconjugated (orb5869) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Blank control (black line): A431. Primary Antibody (green line): Rabbit Anti-CK10 antibody (orb5869), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Blank control: Hela. Primary Antibody (green line): Rabbit Anti-CK10 antibody (orb5869), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Tissue/Cell: rat stomach tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CK10 Polyclonal Antibody, Unconjugated (orb5869) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
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CK10 Rabbit Polyclonal Antibody抗体，orb5869，biorbyt
￥3120
