orb19804 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). This data is from a previous batch, not on sale.
orb19804 (8µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
orb19804 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
orb19804 (0.03µg/ml) staining of Mouse Adrenal Gland lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Western blot analysis of Mouse Heart lysate using PNPLA3 antibody
Immunohistochemical analysis of Human Kidney using PNPLA3 antibody
Immunohistochemical analysis of Human Kidney using PNPLA3 antibody
Immunofluorescence analysis of HepG2 cells using PNPLA3 antibody