IFN Gamma中和抗体， IFN Gamma Monoclonal antibody

IFN Gamma中和抗体， IFN Gamma Monoc

lonal antibody

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价格￥2800

品牌PTGCN
产地美国
货号69007-1-Ig
更新日期2025年07月15日

宿主小鼠

上海研卉生物科技有限公司

11 年金牌会员

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技术资料

库存：
10
靶点：
69007-1-Ig
抗体英文名：
IFN Gamma Monoclonal antibody
抗体名：
IFN Gamma中和抗体
宿主：
小鼠
规格：
100ul

IFN Gamma中和抗体， IFN Gamma Monoclonal antibody

Tested Applications:

Neutralization, ELISA
Application not tested? Please see our Guarantee

Neutralization experiment of NeutraKine® IFN Gamma using 69007-1-Ig

Recombinant human IFN-gamma (Cat.NO. HZ-1301) inhibits the growth of HT-29 cell line (human colorectal adenocarcinoma cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IFN-gamma (5 ng/mL HZ-1301) is neutralized by mouse anti-human IFN-gamma monoclonal antibody 69007-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 100-400 ng/mL.

Neutralization experiment of NeutraKine® IFN Gamma using 69007-1-Ig

Recombinant human IFN-gamma (Cat.NO. HZ-1301) inhibits HT-29 cell line (human colorectal adenocarcinoma cell line) proliferation in the prescence of 0.5 ng/mL TNF alpha in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IFN-gamma (5 ng/mL HZ-1301 axis) is neutralized by mouse anti-human IFN-gamma monoclonal antibody 69007-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 100-500 ng/mL. The NeutraControl mouse anti-human IFN-gamma monoclonal antibody 69507-1-Ig could recognize human IFN-gamma but could not neutralize human IFN-gamma in the range of 0.00244-40 μg/mL (green curve, refer to top X-right Y axis).

ELISA experiment of Recombinant protein using 69007-1-Ig

Indirect ELISA was carried out by coating recombinant Human IFN gamma (Cat.NO. HZ-1301) at 70 ng/well followed by blocking and adding serial diluted IFN gamma antibody 69007-1-Ig and 69507-1-Ig respectively. Signal was developed with TMB and stopped by H2O4. Signal strength was measured by absorbance at 450 nm.

Species Specificity:

Human

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IFN Gamma中和抗体， IFN Gamma Monoclonal antibody

￥2800

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