NR3C2/Mineralocorticoid receptor Rabbit Polyclonal Antibody抗体,orb13588,biorbyt

NR3C2/Mineralocorticoid recept

or Rabbit Polyclonal Antibody抗体,orb13588,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb13588
  • 英国
  • 2025年12月03日
  • FC, IF, IHC-Fr, IHC-P
  • Rabbit
  • Human, Mouse
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    • 详细信息
    • 技术资料
    • 抗体名

      NR3C2/Mineralocorticoid receptor Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      NR3C2/Mineralocorticoid receptor Rabbit Polyclonal Antibody

    • 靶点

      NR3C2

    • 浓度

      1mg/ml

    • 应用范围

      FC, IF, IHC-Fr, IHC-P

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb13588

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      参考产品说明书

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human Mineralocorticoid receptor (601-700/984aa)

    • 规格

      50 ul

    产品描述:NR3C2/Mineralocorticoid receptor Rabbit Polyclonal Antibody

    别名:Aldosterone receptor; MCR; MGC133092; MLR; MR; NR3 C2; NR3C2; NR3C2 protein; Nuclear receptor subfamily 3 group C member 2; MCR_HUMAN.

    免疫原:KLH conjugated synthetic peptide derived from human Mineralocorticoid receptor (601-700/984aa)

    预测反应性:Bovine, Canine, Gallus, Porcine, Rabbit, Rat

    分子量:107 kDa

    应用稀释比例:IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P08235

    Note:For research use only.

    NR3C2/Mineralocorticoid recept
    Blank control: Molt-4. Primary Antibody (green line): Rabbit Anti-RPS6KB1 antibody (orb13588), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    NR3C2/Mineralocorticoid recept
    Fixative-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NR3C2/Mineralocorticoid receptor) Polyclonal Antibody, Unconjugated (orb13588) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

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