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IL-6R alpha Rabbit Polyclonal

Antibody抗体，orb13507，biorbyt
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  • ￥3120
  • biorbyt已认证
  • orb13507
  • 英国
  • 2025年12月17日
  • IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      IL-6R alpha Rabbit Polyclonal Antibody抗体

    • 抗体英文名：

      IL-6R alpha Rabbit Polyclonal Antibody

    • 靶点：

      IL6R

    • 浓度：

      1mg/ml

    • 应用范围：

      IF, IHC-Fr, IHC-P, WB

    • 宿主：

      Rabbit

    • 适应物种：

      Human

    • 保质期：

      6-12个月

    • 抗原来源：

      详询

    • 目录编号：

      orb13507

    • 级别：

      科研级

    • 库存：

      88

    • 供应商：

      biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Polyclonal

    • 保存条件：

      参考产品说明书

    • 形态：

      Liquid

    • 亚型：

      IgG

    • 免疫原：

      KLH conjugated synthetic peptide derived from mouse IL-6R alpha (351-460/460aa)

    • 规格：

      50 ul

    产品描述：IL-6R alpha Rabbit Polyclonal Antibody

    别名:Interleukin-6 receptor subunit alpha; IL6R; CD 126; CD126; CD126 antigen; gp80; IL 6R 1; IL 6R alpha; IL 6R; IL-6 receptor subunit alpha; IL-6R 1; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; Il6r; IL6RA; IL6RA_HUMAN; Interleukin 6 receptor alpha chain; Interleukin 6 receptor; Membrane glycoprotein 80.

    免疫原：KLH conjugated synthetic peptide derived from mouse IL-6R alpha (351-460/460aa)

    预测反应性:Human, Rat

    分子量:50 kDa

    应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P22272

    Note:For research use only.

    产品细节图片1
    Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-IL-6R alpha antibody (orb13507), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片2
    Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-IL-6R alpha antibody (orb13507), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片3
    Blank control: U937. Primary Antibody (green line): Rabbit Anti-IL-6R alpha antibody (orb13507), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells wereincubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片4
    Fixative-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IL-6R alpha) Polyclonal Antibody, Unconjugated (orb13507) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片5
    Fixative-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IL-6R alpha) Polyclonal Antibody, Unconjugated (orb13507) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片6
    Sample: Lane 1: Recombinant Human IL-6R alpha Protein at 50 ng, Lane 2: Recombinant Human IL-6R alpha Protein at 500 ng, Primary: Rabbit Anti-IL-6R alpha Antibody at 1/1000, dilution (Cat.orb13507), Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42kD, Observed band size: 58-75kD.

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    图标文献和实验
    相关实验

    • Anti-HA Immunocytology

      suspended in PBS. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600

    • Anti-HA Immunocytology

      . A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.

    • Immunocytology using the HA epitope

      . A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.

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