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FUT4 Rabbit Polyclonal Antibod

y抗体，orb11420，biorbyt
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  • ￥3120
  • biorbyt已认证
  • orb11420
  • 英国
  • 2025年12月03日
  • FC, IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human, Mouse
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武汉博欧特生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      FUT4 Rabbit Polyclonal Antibody抗体

    • 抗体英文名：

      FUT4 Rabbit Polyclonal Antibody

    • 靶点：

      FUT4

    • 浓度：

      1mg/ml

    • 应用范围：

      FC, IF, IHC-Fr, IHC-P, WB

    • 宿主：

      Rabbit

    • 适应物种：

      Human, Mouse

    • 保质期：

      6-12个月

    • 抗原来源：

      详询

    • 目录编号：

      orb11420

    • 级别：

      科研级

    • 库存：

      88

    • 供应商：

      biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Polyclonal

    • 保存条件：

      参考产品说明书

    • 形态：

      Liquid

    • 亚型：

      IgG

    • 免疫原：

      KLH conjugated synthetic peptide derived from human FUT4 (251-295/433aa)

    • 规格：

      50 ul

    产品描述：FUT4 Rabbit Polyclonal Antibody

    别名:FUT4_HUMAN; alpha-(1, 3)-fucosyltransferase 4; 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; EC:2.4.1.152; ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV(Fuc-TIV; FucT-IV); Galactoside 3-L-fucosyltransferase; ELFT; FCT3A; LeX; CD15; FUTIV; SSEA-1; SSEA1; FUC-TIV;

    免疫原：KLH conjugated synthetic peptide derived from human FUT4 (251-295/433aa)

    预测反应性:Rat

    分子量:58 kDa

    应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P22083

    Note:For research use only.

    产品细节图片1
    Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-FUT4 (orb11420), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片2
    Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-FUT4/FITC Conjugated antibody, Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片3
    Overlay histogram showing HL 60 cells stained with orb11420 (Green line). The cells were fixed with 90% methanol (5 min) and then permeabilized with 0.01M PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (orb11420, 1 µg/1x10^6 cells) for 30 min at 22°C. The secondary antibody used was fluorescein isothiocyanate goat anti-rabbit IgG (H+L) (Brillant blue line) at 1/200 dilution for 30 min at 22°C. Isotype control antibody was rabbit IgG (polyclonal, Orange line) (1 µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of 20000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    产品细节图片4
    Fixative-fixed, paraffin embedded (Human lung cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (FUT4) Polyclonal Antibody, Unconjugated (orb11420) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片5
    Sample: A549 Cell (Human) Lysate at 40 ug Primary: Anti-FUT4 (orb11420) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58kD Observed band size: 63kD.

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