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PDGFRA Rabbit Polyclonal Antib

ody抗体,orb11256,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb11256
  • 英国
  • 2025年12月03日
  • FC, IF, IHC-Fr, IHC-P
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      PDGFRA Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      PDGFRA Rabbit Polyclonal Antibody

    • 靶点

      PDGFRA

    • 浓度

      1mg/ml

    • 应用范围

      FC, IF, IHC-Fr, IHC-P

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb11256

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      参考产品说明书

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human PDGF-R-A (1021-1089/1089aa)

    • 规格

      50 ul

    产品描述:PDGFRA Rabbit Polyclonal Antibody

    别名:Platelet–dirived growth factor receptor-alpha; PDGF Receptor alpha; alpha platelet derived growth factor receptor; CD 140a; CD140a; CD140a antigen; MGC74795; PDGF alpha chain; PDGF R alpha; PDGFR 2; PDGFR A; PDGFR alpha; PDGFR2; Platelet derived growth factor receptor 2; Platelet derived growth factor receptor alpha polypeptide; PGFRA_HUMAN.

    免疫原:KLH conjugated synthetic peptide derived from human PDGF-R-A (1021-1089/1089aa)

    预测反应性:Bovine, Canine, Equine, Gallus, Porcine

    分子量:117 kDa

    应用稀释比例:IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Pubmed:29505751

    UniProt ID:P16234

    Note:For research use only.

    产品细节图片1
    Black line: Positive blank control (SH-Sy5Y), Negative blank control (A431), Green line: Primary Antibody (Rabbit Anti-PDGFRA antibody (orb11256)), Orange line: Isotype Control Antibody (Rabbit IgG). Blue line: Secondary Antibody (Goat anti-rabbit IgG-AF488), SH-Sy5Y (Positive) and A431 (Negative control) cells (black) were fixed with 4% PFA for 10 min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20°C, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PDGFRA Antibody (orb11256) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody (blue) incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

    产品细节图片2
    Blank control: Mouse Kidney (blue). Primary Antibody: Rabbit Anti-PDGFRA antibody (orb11256, Green), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% Fixative for 10 min at 37°C. Primary antibody (orb11256, 1 µg/1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min on ice. Acquisition of 20000 events was performed.

    产品细节图片3
    Tissue/Cell: human colon carcinoma, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PDGFRA Polyclonal Antibody, Unconjugated (orb11256) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    图标文献和实验
    该产品被引用文献
    Cehofski, Lasse Jørgensen et al. Dexamethasone intravitreal implant downregulates PDGFR-α and upregulates caveolin-1 in experimental branch retinal vein occlusion Exp Eye Res, 171, 174-182 (2018)
    相关实验
    • Polyclonal Antibody Production

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    • Polyclonal Antibody Production

        Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation.     Rabbit Immunization Initial Preparation Your antigen

    • Preparation of Polyclonal Antibodies to Retinoid Receptors

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    图标技术资料

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