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NCX1 Rabbit Polyclonal Antibod

y抗体，orb11105，biorbyt
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  • ￥3120
  • biorbyt已认证
  • orb11105
  • 英国
  • 2025年12月03日
  • FC, IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human, Rat
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武汉博欧特生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      NCX1 Rabbit Polyclonal Antibody抗体

    • 抗体英文名：

      NCX1 Rabbit Polyclonal Antibody

    • 靶点：

      SLC8A1

    • 浓度：

      1mg/ml

    • 应用范围：

      FC, IF, IHC-Fr, IHC-P, WB

    • 宿主：

      Rabbit

    • 适应物种：

      Human, Rat

    • 保质期：

      6-12个月

    • 抗原来源：

      详询

    • 目录编号：

      orb11105

    • 级别：

      科研级

    • 库存：

      88

    • 供应商：

      biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Polyclonal

    • 保存条件：

      参考产品说明书

    • 形态：

      Liquid

    • 亚型：

      IgG

    • 免疫原：

      KLH conjugated synthetic peptide derived from human NCX1 (801-900/971aa)

    • 规格：

      50 ul

    产品描述：NCX1 Rabbit Polyclonal Antibody

    别名:Na+/Ca2+exchanger 1; CNC; DKFZp779F0871; MGC119581; FLJ37694; FLJ43417; Na+/Ca2+exchange protein 1; Na+/Ca2+exchanger; NCX 1; NCX; SLC8A1; SLC8A1 protein; Sodium Calcium Exchanger; Sodium/calcium exchanger 1; Solute carrier family 8 member 1; NAC1_HUMAN.

    免疫原：KLH conjugated synthetic peptide derived from human NCX1 (801-900/971aa)

    预测反应性:Bovine, Canine, Equine, Gallus, Guinea pig, Mouse, Porcine, Sheep

    分子量:106 kDa

    应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2ug/test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P32418

    Note:For research use only.

    产品细节图片1
    Blank control: U937. Primary Antibody (green line): Rabbit Anti-NCX1 antibody (orb11105), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片2
    Fixative-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NCX1) Polyclonal Antibody, Unconjugated (orb11105) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

    产品细节图片3
    Sample: 293T (Human) Cell Lysate at 30 ug, HepG2 (Human) Cell Lysate at 30 ug, Panc-1 (Human) Cell Lysate at 30 ug, Primary: Anti-NCX1 (orb11105) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 106 kD, Observed band size: 118 kD.

    产品细节图片4
    Sample: HL-60 (Human) Cell Lysate at 30 ug, K562 (Human) Cell Lysate at 30 ug, Primary: Anti-NCX1 (orb11105) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 106 kD, Observed band size: 118 kD.

    产品细节图片5
    Sample: Lane 1: U251 (Human) Cell Lysate at 30 ug, Lane 2: A431 (Human) Cell Lysate at 30 ug, Lane 3: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-NCX1 (orb11105) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 120 kD, Observed band size: 120 kD.

    产品细节图片6
    U-937 cells were fixed with 4% PFA for 10 min at room temperature, permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CXCL2 Antibody (orb11105) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

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