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- SignaGen
- 美国
- SL100489-C6
- 2026年05月01日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
一年
- 英文名：
GenJet™ In Vitro DNA Tranfection Reagent for C6 Glioma Cells
- 库存：
现货 大量
- 供应商：
济南美中清水湾生物科技有限公司
- 保存条件：
4°C
- 规格：
毫升/支
GenJet™ DNA In Vitro Tranfection Reagent for C6 Glioma cell is pre-optimized for transfecting C6 glioma cells. The C6 glioma cell line is a widely used cell line in neurobiological research. Compared to primary neural cells, the C6 cell line offers some major advantages like easy accessibility and culture as well as a good availability of high cell numbers. Nevertheless, the biggest disadvantage of the C6 cells is that they are difficult-to-transfect with commonly used non-viral transfection methods. The pre-optimized transfection reagent simplifies the transfection of C6 cells. Transfection efficiencies of over 60% can be easily achieved with excellent cell viabilities of above 90%.
Refer to the following optimal transfection conditions for maximal transfection efficiency on C6 glioma cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
| Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium After Transfection Maximal Efficiency Transfection Results: Reporter Gene Plasmid Efficiency (GFP %) |
~80% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes Yes, ~5 Hours After Transfection 48 hours EGFP pEGFP-N3 (CMV promoter) 60% |
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet
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文献和实验The Use of Differentiating N2a and C6 Cell Lines for Studies of Organophosphate Toxicity
and molecular end points in differentiating mammalian cell lines. This chapter describes the key practical approaches that we use to monitor OP toxicity in mouse N2a neuroblastoma and rat C6 glioma cell lines. These cell lines are easy
【原创】Lipofectamine? 2000 promega
更多详情可登陆上海普迈生物科技有限公司http://www.retbio.com 多种转染试剂满足您的特定的cDNA和shRNA转染需求。 Cell Types Examples Recommended Reagents Commonly
, 4(3):125 29 Kondo T, Setoguchi T, Taga T. Persistence of a small subpopulation of cancer stem-like cells in the C6 glioma cell line. Proc Natl Acad Sci U S A. 2004 Jan 20;101(3):781-6. 30 Haraguchi N, Utsunomiya T, Inoue H, Tanaka F, Mimori K, Barnard GF
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