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GLUT4 Rabbit Polyclonal Antibo

dy抗体,orb10728,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb10728
  • 英国
  • 2025年12月03日
  • FC, ICC, IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      GLUT4 Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      GLUT4 Rabbit Polyclonal Antibody

    • 靶点

      SLC2A4

    • 浓度

      1mg/ml

    • 应用范围

      FC, ICC, IF, IHC-Fr, IHC-P, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb10728

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      参考产品说明书

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human GLUT4 (401-509/509aa)

    • 规格

      50 ul

    产品描述:GLUT4 Rabbit Polyclonal Antibody

    别名:insulin-responsive; Glucose transporter GLUT 4; Glucose Transporter GLUT4; Glucose transporter type 4; Glucose transporter type 4 insulin responsive; GLUT 4; GLUT-4; GTR4_HUMAN; kug; SLC 2A4; SLC2A4; solute carrier family 2(facilitated glucose transporter) member 4; Solute carrier family 2, facilitated glucose transporter member 4.

    免疫原:KLH conjugated synthetic peptide derived from human GLUT4 (401-509/509aa)

    预测反应性:Bovine, Canine, Porcine, Rabbit, Sheep

    分子量:54 kDa

    应用稀释比例:WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P14672

    Note:For research use only.

    产品细节图片1
    Blank control (blue line): K562 (blue). Primary Antibody (green line): Rabbit Anti-GLUT4 antibody (orb10728), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were fixed with 70% ethanol (overnight at 4°C) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片2
    Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-GLUT4 (orb10728) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.

    产品细节图片3
    Sample: Heart (Rat) Cell Lysate at 40 ug, Primary: Anti-GLUT4 (orb10728) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.

    产品细节图片4
    Sample: Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 2: Heart (Mouse) Lysate at 40 ug, Lane 3: Heart (Rat) Lysate at 40 ug, Lane 4: Muscle (Mouse) Lysate at 40 ug, Lane 5: Muscle (Rat) Lysate at 40 ug, Primary: Anti-GLUT4 (orb10728) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kD, Observed band size: 51 kD.

    产品细节图片5
    Tissue/Cell: NIH/3T3 cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (GLUT4) polyclonal Antibody, Unconjugated (orb10728) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

    产品细节图片6
    Tissue/Cell: rat kidney tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GLUT4 Polyclonal Antibody, Unconjugated (orb10728) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    图标文献和实验
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