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DNA Ligase IV Rabbit Polyclona

l Antibody抗体，orb10564，biorbyt
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  • ￥3120
  • biorbyt已认证
  • orb10564
  • 英国
  • 2025年12月03日
  • IF, IHC-Fr, IHC-P
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      DNA Ligase IV Rabbit Polyclonal Antibody抗体

    • 抗体英文名：

      DNA Ligase IV Rabbit Polyclonal Antibody

    • 靶点：

      LIG4

    • 浓度：

      1mg/ml

    • 应用范围：

      IF, IHC-Fr, IHC-P

    • 宿主：

      Rabbit

    • 适应物种：

      Human

    • 保质期：

      6-12个月

    • 抗原来源：

      详询

    • 目录编号：

      orb10564

    • 级别：

      科研级

    • 库存：

      88

    • 供应商：

      biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Polyclonal

    • 保存条件：

      参考产品说明书

    • 形态：

      Liquid

    • 亚型：

      IgG

    • 免疫原：

      KLH conjugated synthetic peptide derived from human DNA Ligase IV (51-150/911aa)

    • 规格：

      50 ul

    产品描述：DNA Ligase IV Rabbit Polyclonal Antibody

    别名:LIG4; DNA ligase IV ATP dependent; DNA joinase; DNA joinase; DNA ligase 4; DNA ligase IV; DNA repair enzyme; DNA repair enzyme; DNLI4_HUMAN; LIG 4; LIG4; LIG-4; Ligase IV; Ligase IV; Ligase IV DNA ATP dependent; Polydeoxyribonucleotide synthase [ATP] 4; Polydeoxyribonucleotide synthase 4; Polydeoxyribonucleotide synthase; Polydeoxyribonucleotide synthase; Polynucleotide ligase; Polynucleotide ligase; Sealase.

    免疫原：KLH conjugated synthetic peptide derived from human DNA Ligase IV (51-150/911aa)

    预测反应性:Bovine, Canine, Equine, Gallus, Mouse, Rabbit, Rat, Sheep

    分子量:104 kDa

    应用稀释比例：IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P49917

    Note:For research use only.

    产品细节图片1
    paraffin embedded (human testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (DNA Ligase IV) Polyclonal Antibody, Unconjugated (orb10564) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片2
    Tissue/Cell: human laryngocarcinoma, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-LIG4 Polyclonal Antibody, Unconjugated (orb10564) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    图标文献和实验
    相关实验

    • OGMP - Organelle DNA Library Construction

      . PHOSPHORYLATION 10 ul sized DNA 2 ul rATP (stock : 10 mM) 2 ul ligase-buffer 10x (similar to kinase buffer) 5 units kinase --------------- in 20 ul final volume Incubate for 30 min at 37'C

    • 细胞器DNA文库构建

      -elute 2) fractions (0.5 - 1.0 kbp and 1.0 - 3 kbp; ligate separately) 2. Add glycogen (1 ul of 20mg/ml stock), precipitate by EtOH/AmAc (spin down for 30 min). Dissolve pellet in 10 ul TE.IV. PHOSPHORYLATION 10 ul sized DNA

    • In Vitro Rejoining of Double Strand Breaks in Genomic DNA

      including DNA-PKcs, Ku, DNA ligase IV, XRCC4, XLF/Cernunnos, and Artemis. Nevertheless, there is evidence that as of yet uncharacterized repair factors may contribute to the efficiency of NHEJ, for example by modulating the activity of known factors

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