- ￥3120
- biorbyt
- orb10256
- 英国
- 2025年12月03日
- FC, IF, IHC-Fr, IHC-P, WB
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
RUNX2 Rabbit Polyclonal Antibody抗体
- 抗体英文名：
RUNX2 Rabbit Polyclonal Antibody
- 靶点：
RUNX2
- 浓度：
1mg/ml
- 应用范围：
FC, IF, IHC-Fr, IHC-P, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb10256
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
参考产品说明书
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human RUNX2 (202-300/521aa)
- 规格：
50 ul
产品描述：RUNX2 Rabbit Polyclonal Antibody
别名:RUNX2_HUMAN; Runx-2; Runt-related Transcription Factor 2; CBF alpha 1; CBF-alpha-1; PEBP2-alpha A; CBFA1; CCD; CCD1; Cleidocranial dysplasia 1; Core binding factor; Core binding factor runt domain alpha subunit 1; Core binding factor subunit alpha 1; MGC120023; Oncogene AML 3; OSF 2; OSF2; OSF-2; Osteoblast specific transcription factor 2; OTTHUMP00000016533; PEA2 alpha A; PEA2aA; PEBP2 alpha A; PEBP2A1; PEBP2A2; PEBP2aA1; Polyomavirus enhancer binding protein 2 alpha A subunit; Runt domain; Runt related transcription factor 2; SL3 3 enhancer factor 1 alpha A subunit; SL3/AKV core binding factor alpha A subunit; AML3; CLCD.
免疫原：KLH conjugated synthetic peptide derived from human RUNX2 (202-300/521aa)
预测反应性:Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep
分子量:57(hu)/67(mo,rat) kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Pubmed:22955562, 28874877
UniProt ID:Q13950
Note:For research use only.
别名:RUNX2_HUMAN; Runx-2; Runt-related Transcription Factor 2; CBF alpha 1; CBF-alpha-1; PEBP2-alpha A; CBFA1; CCD; CCD1; Cleidocranial dysplasia 1; Core binding factor; Core binding factor runt domain alpha subunit 1; Core binding factor subunit alpha 1; MGC120023; Oncogene AML 3; OSF 2; OSF2; OSF-2; Osteoblast specific transcription factor 2; OTTHUMP00000016533; PEA2 alpha A; PEA2aA; PEBP2 alpha A; PEBP2A1; PEBP2A2; PEBP2aA1; Polyomavirus enhancer binding protein 2 alpha A subunit; Runt domain; Runt related transcription factor 2; SL3 3 enhancer factor 1 alpha A subunit; SL3/AKV core binding factor alpha A subunit; AML3; CLCD.
免疫原：KLH conjugated synthetic peptide derived from human RUNX2 (202-300/521aa)
预测反应性:Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep
分子量:57(hu)/67(mo,rat) kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Pubmed:22955562, 28874877
UniProt ID:Q13950
Note:For research use only.
Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-RUNX2 antibody (orb10256), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 1 µg/test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Fixative-fixed, paraffin embedded (Mouse thyroid), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RUNX2) Polyclonal Antibody, Unconjugated (orb10256) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Large intestine (Mouse) Lysate at 40 ug, Lane 2: Lymph node (Mouse) Lysate at 40 ug, Primary: Anti-RUNX2 (orb10256) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60-65 kD, Observed band size: 60 kD.
Sample: Lane 1: Mouse Colon tissue lysates, Lane 2: Mouse Lymph node tissue lysates, Lane 3: Rat Colon tissue lysates, Lane 4: Rat Lymph node tissue lysates, Primary: Anti-RUNX2 (orb10256) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57/67 kDa, Observed band size: 60 kDa.
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文献和实验该产品被引用文献
Zhang, Ping et al. Contribution of SATB2 to the stronger osteogenic potential of bone marrow stromal cells from craniofacial bones Cell Tissue Res, 350, 425-437 (2012), Zhang, Ci et al. Bone regeneration in minipigs by intrafibrillarly-mineralized collagen loaded with autologous periodontal ligament stem cells Sci Rep, 7, 10519 (2017)
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RUNX2 Rabbit Polyclonal Antibody抗体，orb10256，biorbyt
￥3120
