重组食蟹猴 RANKL / OPGL / TNFSF11 蛋白 (Fc标签)

重组食蟹猴 RANKL / OPGL / TNFSF11 蛋白 (Fc标签)（产品说明）
蛋白名称：Cynomolgus RANKL / OPGL / TNFSF11 Protein (Fc Tag)
蛋白构建：A DNA sequence encoding the cynomolgus TNFSF11 (G7PW41) (Gly136-Asp317) was expressed with the Fc region of human IgG1 at the N-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 85 % as determined by SDS-PAGE
蛋白活性：1. Immobilized Cynomolgus S4-Fc3L3-TNFSF11 at 10 μg/ml (100 μl/well) can bind biotinylated human TNFRSF11B-His (Cat:10271-H08H), The EC50 of biotinylated human TNFRSF11B-His (Cat:10271-H08H) is 7.94-18.52 ng/ml. 2. The bioactivity of RANKL was determined by measuring the ability of RANKL to induce TRAP activity in RAW264.7 cells. The ED50 for this effect is typically 7-35 ng/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Glu
蛋白分子量：The recombinant cynomolgus TNFSF11 comprises 442 amino acids and has a calculated molecular mass of 48.9 KDa. The apparent molecular mass of the protein is approximately 55 KDa in SDS-PAGE.
蛋白NP号：G7PW41
蛋白氨基酸序列：Gly136-Asp317
蛋白标签：N-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.