重组人 HGF / Hepatocyte Growth Factor 蛋白(产品说明)
蛋白名称:Human HGF / Hepatocyte Growth Factor Protein
蛋白构建:A DNA sequence encoding the human HGF (NP_000592.3) precursor (Met 1-Ser 728) was expressed and purified.
表达宿主:CHO Stable Cells
蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性:Measured by its ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell proliferation. The ED50 for this effect is typically 0.2-2 ng/mL.
蛋白内毒素:< 0.01 EU per μg of the protein.
预测N端:Gln 32 (α chain) & Val 495 (β chain)
蛋白分子量:The secreted recombinant human HGF consists of 697 amino acids after cleavage of the signal peptide and has a predicted molecular mass of 79.7 kDa. The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain. As a result of glycosylation, it migrates with the apparent molecular mass of 90, 60 and 34 kDa corresponding to the single chain, α chain and β chain respectively in SDS-PAGE under reducing conditions.
蛋白NP号:NP_000592.3
蛋白氨基酸序列:Met1-Ser728
蛋白标签:Native
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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