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ADAMTS7 (NT) Rabbit Polyclonal

Antibody抗体,orb10042,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb10042
  • 英国
  • 2025年12月02日
  • IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      ADAMTS7 (NT) Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      ADAMTS7 (NT) Rabbit Polyclonal Antibody

    • 靶点

      ADAMTS7

    • 浓度

      1mg/ml

    • 应用范围

      IF, IHC-Fr, IHC-P, WB

    • 宿主

      Rabbit

    • 适应物种

      Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb10042

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human ADAMTS7 (241-340/1595aa)

    • 规格

      50 ul

    产品描述:ADAMTS7 (NT) Rabbit Polyclonal Antibody

    别名:A disintegrin and metalloproteinase with thrombospondin motifs 7; ADAM metallopeptidase with thrombospondin type 1 motif 7; A disintegrin and metalloprotease with thrombospondin motifs 7 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 7; A disintegrin like and metalloprotease(reprolysin type) with thrombospondin type 1 motif 7; A disintegrin like and metalloprotease with thrombospondin type 1 motif 7; ADAM metallopeptidase with thrombospondin type 1 motif 7; ADAM metallopeptidase with thrombospondin type 1 motif 7 preproprotein; ADAM TS 7; ADAM TS7; ADAMTS 7; ADAMTS7; COMPase; DKFZp434H204; ATS7_MOUSE.

    免疫原:KLH conjugated synthetic peptide derived from human ADAMTS7 (241-340/1595aa)

    预测反应性:Human

    分子量:158 kDa

    应用稀释比例:WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Pubmed:25133350

    UniProt ID:Q6P7J9

    Note:For research use only.

    产品细节图片1
    paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ADAMTS7 (NT)) Polyclonal Antibody, Unconjugated (orb10042) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片2
    Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-ADAMTS7 (NT) (orb10042) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 158 kD, Observed band size: 158 kD.

    产品细节图片3
    Tissue/cell: rat brain tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-ADAMTS7 Polyclonal Antibody, Unconjugated (orb10042) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

    产品细节图片4
    Tissue/cell: rat brain tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-ADAMTS7 Polyclonal Antibody, Unconjugated (orb10042) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

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    图标文献和实验
    该产品被引用文献
    Bond, Andrew R. et al. Cartilage oligomeric matrix protein (COMP) in murine brachiocephalic and carotid atherosclerotic lesions Atherosclerosis, 236, 366-372 (2014)
    相关实验
    • Fluorescent in situ Hybridization Combined with Immunostaining on Polytene Chromosomes

      . for most rabbit polyclonal antibodies, dilutions are usually between 1:30 and 1:500). Cover with coverslip and incubate overnight at 40°C in a humid chamber; After the overnight incubation, rinse 2x5 minutes in PBS 5 minutes at RT; Wash 15 minutes

    • (共享)免疫单词缩写及翻译

      -rabbit serum 羊抗兔血清 GCA gastrin cell antibody 胃泌素细胞抗体 GFAP glial fibrillary acidic protein 胶质纤维酸性蛋白 GFL green fluorescence 绿色荧光 GH growth hormone 生素激素 GLAD gold-labelled antigen detection method 金标抗原检测法 GMA glycol methacrylate

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