AGEs Rabbit Polyclonal Antibody抗体,orb10064,biorbyt

AGEs Rabbit Polyclonal Antibod

y抗体,orb10064,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb10064
  • 英国
  • 2025年12月02日
  • FC, IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 技术资料
    • 抗体名

      AGEs Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      AGEs Rabbit Polyclonal Antibody

    • 靶点

      SERPINB14

    • 浓度

      1mg/ml

    • 应用范围

      FC, IF, IHC-Fr, IHC-P, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb10064

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      AGEs

    • 规格

      50 ul

    产品描述:AGEs Rabbit Polyclonal Antibody

    别名:advanced glycosylation end products; AGE; Advanced glycation end-products;

    免疫原:AGEs

    预测反应性:Human, Mouse, Rat

    分子量:62 kDa

    应用稀释比例:WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Note:For research use only.

    AGEs Rabbit Polyclonal Antibod
    Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-AGEs antibody (orb10064), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    AGEs Rabbit Polyclonal Antibod
    Sample: Lane 1: Kidney (Mouse) Tissue Lysate at 40 ug, Lane 2: Lung (Mouse) Tissue Lysate at 40 ug, Lane 3: Large intestine (Mouse) Tissue Lysate at 40 ug, Lane 4: Testis (Rat) Tissue Lysate at 40 ug, Lane 5: Lung (Rat) Tissue Lysate at 40 ug, Lane 6: Large intestine (Rat) Tissue Lysate at 40 ug, Lane 7: U87MG (Human) Cell Lysate at 30 ug. Primary: Anti-AGEs (orb10064) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Observed band size: 62 kD.

    AGEs Rabbit Polyclonal Antibod
    Tissue/cell: Rat kidney tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-AGEs Polyclonal Antibody, Unconjugated (orb10064) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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