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Phospho-Nrf2 (Ser40) Rabbit Po

lyclonal Antibody抗体，orb6544，biorbyt
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  • ￥3120
  • biorbyt已认证
  • orb6544
  • 英国
  • 2025年12月03日
  • ICC, WB
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      Phospho-Nrf2 (Ser40) Rabbit Polyclonal Antibody抗体

    • 抗体英文名：

      Phospho-Nrf2 (Ser40) Rabbit Polyclonal Antibody

    • 靶点：

      NFE2L2

    • 浓度：

      1mg/ml

    • 应用范围：

      ICC, WB

    • 宿主：

      Rabbit

    • 适应物种：

      Human

    • 保质期：

      6-12个月

    • 抗原来源：

      详询

    • 目录编号：

      orb6544

    • 级别：

      科研级

    • 库存：

      88

    • 供应商：

      biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Polyclonal

    • 形态：

      Liquid

    • 亚型：

      IgG

    • 免疫原：

      KLH conjugated Synthesised phosphopeptide derived from human Nrf2 around the phosphorylation site of Ser40 DF(p-S)QR

    • 规格：

      50 ul

    产品描述：Phospho-Nrf2 (Ser40) Rabbit Polyclonal Antibody

    别名:NF2L2_HUMAN; Nuclear factor erythroid 2-related factor 2; NFE2L2; NF-E2-related factor 2; NFE2-related factor 2; Nrf-2; Nrf 2; Nuclear factor, erythroid derived 2, like 2; NFE2L2; NRF2; NFE2 like bZIP transcription factor 2; HEBP1; IMDDHH;

    免疫原：KLH conjugated Synthesised phosphopeptide derived from human Nrf2 around the phosphorylation site of Ser40 DF(p-S)QR

    预测反应性:Bovine, Canine, Gallus, Mouse, Rat

    分子量:68 kDa

    应用稀释比例：WB=1:500-2000, ICC/IF=1:100-500

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Pubmed:39312096, 40299297, 40999314, 26363155

    UniProt ID:Q16236

    Note:For research use only.

    产品细节图片1
    Blank control (blue line): Jurkat (fixed with 2% Fixative (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-phospho-Nrf2 (Ser40) antibody (orb6544), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

    产品细节图片2
    HepG2 cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-Nrf2 (Ser40)) polyclonal Antibody, Unconjugated (orb6544) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

    产品细节图片3
    Fixative-fixed, paraffin embedded (Mouse brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (phospho-Nrf2 (Ser40)) Polyclonal Antibody, Unconjugated (orb6544) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

    产品细节图片4
    Fixative-fixed, paraffin embedded (Rat brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (phospho-Nrf2 (Ser40)) Polyclonal Antibody, Unconjugated (orb6544) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

    产品细节图片5
    Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: HepG2 (Human) Cell Lysate at 30 ug, Lane 3: MCF-7 (Human) Cell Lysate at 30 ug, Lane 4: Jurkat (Human) Cell Lysate at 30 ug, Lane 5: B16 (Human) Cell Lysate at 30 ug, Lane 6: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-Nrf2 (Ser40) (orb6544) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kD, Observed band size: 135 kD.

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    图标文献和实验
    该产品被引用文献
    Helia Aghamiri # 1 2, Afrooz Mohammadgholi-Beiki # 3, Rojin Rashidian 1 3, Manijeh Motevalian 1 3, Parvaneh Rahimi-Moghaddam 3, Mohammad Sheibani # 4 5, Majid Jafari-Sabet Zhumeria majdae essential oil attenuates TNBS-induced colitis in rats by regulating inflammatory and apoptotic pathways Inflammopharmacology, (2024), Afrooz Mohammadgholi-Beiki 1, Helia Aghamiri 2, Rojin Rashidian 1, Majid Jafari-Sabet 1 3, Manijeh Motevalian 1 3, Parvaneh Rahimi-Moghaddam 4, Mohammad Sheibani Investigation of the Protective Effects of Aripiprazole on Methylphenidate-induced Neurotoxicity in Rats Mol Neurobiol, (2025), Aida Sabouri 1 2, Abbasali Gaeini 2, Soheila Adeli 1, Alireza Ghardashi Afousi 2 High-intensity interval training attenuates cardiac injury by targeting ferroptosis and endoplasmic reticulum-stress in male rats with heart failure Physiol Rep, (2025), Gupta, Mahesh et al. Dwindling of cardio damaging effect of isoproterenol by Punica granatum L. peel extract involve activation of nitric oxide-mediated Nrf2/ARE signaling pathway and apoptosis inhibition Nitric Oxide, 50, 105-113 (2015)
    相关实验

    • Polyclonal Antibody Production

      to the pre-bleed, but get at least 20 mL per rabbit, with 40 mL being the upper limit for an adult rabbit (any more and they get anemic). Monitoring of Titer The quality of the antibodies in serum (humoral immune response) of the bleeds

    • Polyclonal Antibody Production

        Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation.     Rabbit Immunization Initial Preparation Your antigen

    • Polyclonal Antibody Production 简单快捷制备多抗方法，可用于western、ELISA和免疫/b

      the process for the other rabbits. Injections will be done every 4-6 weeks, with bleeds 7-10 days after each injection. Bleeds are performed identically to the pre-bleed, but obtain at least 20 mL per rabbit, with 40 mL being the upper limit for an adult

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    Phospho-Nrf2 (Ser40) Rabbit Polyclonal Antibody抗体，orb6544，biorbyt
    ￥3120