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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Polyprenol mix C70-C85
- 库存:
1
- 供应商:
甄准生物
- 规格:
5mg
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文献和实验(1992) The oligonucleotides we use are kits, contaning 20 decamers, from Operon Technologies Inc. . I. DNase I treatment of RNA mix and incubate for 30 min. at 37 °C : 50 µgRNA, 10 µl RNase inhibitor (1 U/µl), 1 µl
at 30 °C (shaking or not shaking) add 85 µl DMSO heat shock at 42 °C for 7 min spin, resuspend cells in 1 ml 1 x TE spin, resuspend cells in 0.5 ml 1 x TE plate 100 - 200 µl / plate and incubate at 30 °C
The ribonuclease protection assay (RPA)
P]UTP 1 µl T7 RNA polymerase (Keep at -20°C until use, return to -20°C immediately). Mix by gentle pipetting or flicking and quick spin in a microfuge. Incubate at 37°C for 1 hour. 2. Terminate the reaction by adding 2 µl of DNase. Mix
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