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文献和实验Yeast IF with Methanol/Acetone Dehydration
containing 1.2M sorbitol. Be very gentle with the cells! Resuspend in 0.5 mls sorbitol buffer. The cells can be stored at this point overnight at 4¡C. Zymolyase treat the cells with 30 ul 10 mg/ml Zymolyase (100T) at 30¡C for anywhere between 10-30 min
Yeast IF with Methanol/Acetone Dehydration
. Dissolve as a 1% stock solution in good water. Quick freeze in aliqoutes and store at -80¡C. Same applies as for zymolyase. DAPI Stock solution should be at 1 mg/ml in water and stored at -20¡C. Mount Dissolve 100 mg p
Lipoprotein Isolation and Analysis
the arrangement of lipid and protein moieties Proteins usually have a density of >1.3 g/ml. Since lipids have a lower density than water (oil floats on the surface), the density of lipoproteins depends on the ratio of lipid to protein. Lipoproteins
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