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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
VECTASTAIN ABC Kit (Standard*)
- 供应商：
上海伟进生物科技有限公司
- 保存条件：
4°
- 规格：
KIT
Vector Laboratories公司VECTASTAIN@ ABC免疫组化试剂盒
Vector公司于1977年率先开发出了生物素-亲和素系统，该产品系列是检测方法上的重要革命。其后，公司发展出ABC技术（Avidin : Biotinylated Enzyme Complex Technology，亲和素-生物素-过氧化物酶复合物技术），并建立了著名的VECTASTAIN®ABC系统，目前，该系统被视为最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。ABC技术利用生物素和卵白素具有极高亲和性的生物学特征，将卵白素和生物素化辣根酶按照一定的比例混合，形成ABC复合物。该法亦被称作三步法，第一步为biotin化二抗和一抗结合，第二步为avidin（亲和素，A液）和二抗上的biotin结合，第三步为HRP偶联的biotin（B液）和avidin结合，而HRP再催化底物显色完成染色。
试剂盒组份：
2ml试剂A（Avidin DH溶液）
2ml试剂B（生物素化的酶）
1ml生物素化的二抗（1.5mg anti-IgG/0.5mg anti-IgM抗体/2.1mg通用抗体）
3ml阻断血清
其中standard试剂盒只含有试剂A和试剂B
VECTASTAIN®ABC系列产品
免疫组化封闭剂：
免疫组化底物：
复染剂：
封片剂：
免疫组化笔：
试剂盒组份：
2ml试剂A（Avidin DH溶液）
2ml试剂B（生物素化的酶）
1ml生物素化的二抗（1.5mg anti-IgG/0.5mg anti-IgM抗体/2.1mg通用抗体）
3ml阻断血清
其中standard试剂盒只含有试剂A和试剂B
VECTASTAIN®ABC系列产品
- VECTASTAIN®ABC-HRP Kits（辣根过氧化物酶）最通用的系统，使用范围广
- VECTASTAIN®ABC-AP Kits（碱性磷酸酶）灵敏度较高，染色密度较低，适于形态学染色非常重要的组织
- VECTASTAIN®ABC-GO Kits（葡萄糖氧化酶）灵敏度较低，适用于组织内源性HRP或AP含量比较高的组织，常与HRP或AP系统配合进行双染。
- VECTASTAIN®ABC Kits（辣根过氧化物酶）灵敏度高，特别适用于神经组织
| 供应商 | 产品编号 | 产品名称 | 规格 | RMB报价 |
| Vectorlabs | PK-4000 | VECTASTAIN ABC Kit (Standard*) | 1 Kit | 3120.00 |
| Vectorlabs | PK-4001 | VECTASTAIN ABC Kit (Rabbit IgG ) | 1 Kit | 5200.00 |
| Vectorlabs | PK-4002 | VECTASTAIN ABC Kit (Mouse IgG ) | 1 Kit | 5200.00 |
| Vectorlabs | PK-4003 | VECTASTAIN ABC Kit (Human IgG ) | 1 Kit | 5200.00 |
| Vectorlabs | PK-4004 | VECTASTAIN ABC Kit (Rat IgG) | 1 Kit | 5200.00 |
| Vectorlabs | PK-4005 | VECTASTAIN ABC Kit (Goat IgG ) | 1 Kit | 5200.00 |
| Vectorlabs | PK-4006 | VECTASTAIN ABC Kit (Sheep IgG) | 1 Kit | 5200.00 |
| Vectorlabs | PK-4007 | VECTASTAIN ABC Kit (Guinea Pig IgG ) | 1 Kit | 5200.00 |
| Vectorlabs | PK-4010 | VECTASTAIN ABC Kit (Mouse IgM ) | 1 Kit | 5200.00 |
| Vectorlabs | PK-6100 | VECTASTAIN Elite ABC Kit (Standard*) | 1 Kit | 3440.00 |
| Vectorlabs | PK-6101 | VECTASTAIN Elite ABC Kit (Rabbit IgG) | 1 Kit | 5520.00 |
| Vectorlabs | PK-6102 | VECTASTAIN Elite ABC Kit (Mouse IgG) | 1 Kit | 5520.00 |
| Vectorlabs | PK-6103 | VECTASTAIN Elite ABC Kit (Human IgG) | 1 Kit | 5520.00 |
| Vectorlabs | PK-6104 | VECTASTAIN Elite ABC Kit (Rat IgG) | 1 Kit | 5520.00 |
| Vectorlabs | PK-6105 | VECTASTAIN Elite ABC Kit (Goat IgG) | 1 Kit | 5520.00 |
| Vectorlabs | PK-6106 | VECTASTAIN Elite ABC Kit (Sheep IgG) | 1 Kit | 5520.00 |
| Vectorlabs | PK-6200 | VECTASTAIN Elite ABC Kit (Universal) | 1 Kit | 5920.00 |
| Vectorlabs | PK-7100 | R.T.U. VECTASTAIN Elite ABC Reagent | 50 ml | 2720.00 |
| Vectorlabs | PK-7200 | R.T.U. VECTASTAIN Elite ABC Kit (Universal) | 1 Kit | 3360.00 |
| Vectorlabs | AK-5000 | VECTASTAIN ABC-AP KIT (Standard*) | 1 Kit | 3120.00 |
| Vectorlabs | AK-5001 | VECTASTAIN ABC-AP KIT (Rabbit IgG) | 1 Kit | 5200.00 |
| Vectorlabs | AK-5002 | VECTASTAIN ABC-AP KIT (Mouse IgG) | 1 Kit | 5200.00 |
| Vectorlabs | AK-5003 | VECTASTAIN ABC-AP KIT (Human IgG) | 1 Kit | 5200.00 |
| Vectorlabs | AK-5004 | VECTASTAIN ABC-AP KIT (Rat IgG) | 1 Kit | 5200.00 |
| Vectorlabs | AK-5005 | VECTASTAIN ABC-AP KIT (Goat IgG) | 1 Kit | 5200.00 |
| Vectorlabs | AK-5006 | VECTASTAIN ABC-AP KIT (Sheep IgG) | 1 Kit | 5200.00 |
| Vectorlabs | AK-5007 | VECTASTAIN ABC-AP KIT (Guinea Pig IgG) | 1 Kit | 5200.00 |
| Vectorlabs | AK-5010 | VECTASTAIN ABC-AP KIT (Mouse IgM) | 1 Kit | 5200.00 |
| Vectorlabs | AK-5200 | VECTASTAIN ABC-AP KIT (Universal) | 1 Kit | 5600.00 |
| 供应商 | 产品编号 | 产品名称 | 规格 | RMB报价 |
| Vectorlabs | S-1000 | Normal Goat Serum | 20 ml | 1520.00 |
| Vectorlabs | S-2000 | Normal Horse Serum | 20 ml | 1520.00 |
| Vectorlabs | S-2012-50 | Normal Horse Serum | 50 ml | 1280.00 |
| Vectorlabs | S-3000 | Normal Chicken Serum | 20 ml | 1520.00 |
| Vectorlabs | S-4000 | Normal Swine Serum | 20 ml | 1520.00 |
| Vectorlabs | S-5000 | Normal Rabbit Serum | 20 ml | 1520.00 |
| Vectorlabs | SP-5050 | Bovine Serum Albumin (BSA) | 500 mg | 1520.00 |
| 供应商 | 产品编号 | 产品名称 | 规格 | RMB报价 |
| Vectorlabs | SK-4100 | DAB Substrate Kit | 1 Kit | 2000.00 |
| Vectorlabs | H-2200 | DAB Enhancing Solution | 30 ml | 1600.00 |
| Vectorlabs | SK-4105 | ImmPACT™ DAB | 120 ml | 1840.00 |
| Vectorlabs | SK-4200 | AEC Substrate Kit | 1 Kit | 2000.00 |
| Vectorlabs | SK-4205 | ImmPACT™ AEC | 120 ml | 1840.00 |
| Vectorlabs | SK-4285 | ImmPACT™ AMEC Red | 120 ml | 1840.00 |
| Vectorlabs | SK-4300 | 4-CN Substrate Kit | 1 Kit | 2000.00 |
| Vectorlabs | SK-4400 | TMB Substrate Kit | 1 Kit | 2000.00 |
| Vectorlabs | SK-4500 | ABTS® Substrate Kit | 1 Kit | 2000.00 |
| Vectorlabs | SK-4600 | Vector® VIP Substrate Kit | 1 Kit | 2000.00 |
| Vectorlabs | SK-4605 | ImmPACT™ VIP | 120 ml | 1840.00 |
| Vectorlabs | SK-4700 | Vector® SG Substrate Kit | 1 Kit | 2000.00 |
| Vectorlabs | SK-4705 | ImmPACT™ SG | 120 ml | 1840.00 |
| Vectorlabs | SK-4800 | Vector® NovaRED™ Substrate Kit | 1 Kit | 2000.00 |
| Vectorlabs | SK-4805 | ImmPACT™ NovaRED™ | 120 ml | 1840.00 |
| Vectorlabs | SK-5100 | Vector® Red Substrate Kit | 1 Kit | 2000.00 |
| Vectorlabs | SK-5200 | Vector® Black Substrate Kit | 1 Kit | 2000.00 |
| Vectorlabs | SK-5300 | Vector® Blue Substrate Kit | 1 Kit | 2000.00 |
| Vectorlabs | SK-5400 | BCIP/NBT Substrate Kit IV | 1 Kit | 2000.00 |
| Vectorlabs | SK-5900 | p-Nitrophenylphosphate Concentrate | 18 ml | 1600.00 |
| Vectorlabs | SK-6604-200 | DuoLuX™Chemiluminescent/Fluorescent Substrate | 200 ml | 4000.00 |
| Vectorlabs | SK-6605-100 | DuoLuX™Chemiluminescent/Fluorescent Substrate | 100 ml | 3840.00 |
| 供应商 | 产品编号 | 产品名称 | 规格 | RMB报价 |
| Vectorlabs | H-3401 | Vector® Hematoxylin | 500 ml | 1920.00 |
| Vectorlabs | H-3402 | Vector® Methyl Green | 500 ml | 2160.00 |
| 供应商 | 产品编号 | 产品名称 | 规格 | RMB报价 |
| Vectorlabs | H-1000 | VECTASHIELD® Mounting Medium | 10 ml | 1840.00 |
| Vectorlabs | H-1200 | VECTASHIELD® with DAPI | 10 ml | 2000.00 |
| Vectorlabs | H-1300 | VECTASHIELD® with Propidium Iodide | 10 ml | 2000.00 |
| Vectorlabs | H-1400 | VECTASHIELD® HardSet™ Mounting Medium | 10 ml | 2000.00 |
| Vectorlabs | H-1500 | VECTASHIELD® HardSet™ with DAPI | 10 ml | 2160.00 |
| Vectorlabs | H-5000 | VectaMount™ Permanent Mounting Medium | 60 ml | 1680.00 |
| Vectorlabs | H-5501-60 | VectaMount™AQ Aqueous Mounting Medium | 60 ml | 1600.00 |
| 供应商 | 产品编号 | 产品名称 | 规格 | RMB报价 |
| Vectorlabs | H-4000 | ImmEdge™ Pen Vector免疫组化笔 | 2-Pen Set* | 1760.00 |
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文献和实验相关实验
The vector-mediated introduction of cDNA into mammalian cells by calcium phosphate co-precipitation or permeation with lipofectamine is widely used for the integration of cDNA into genomic DNA. Such integration, however, of cDNA occurs
the secondary antibody at a 1/400 dilution. Apply 150µl and incubate 30 min., RT in a humid chamber. Blot off the antibody, wash 2 times 5 min. each in 1x PBS. Prepare the working dilution of ABC-Vector Red complex from the Vector ABC kit (Catalog No. AK
Bromodeoxyuridine Immunohistochemistry
utilizing VectaStain kits from Vector Laboratories with some alteration to the manufacturer''s instructions. 10. Block 30 min. RT Prepare blocking reagent: add 1 drop of normal horse serum in 3.3 ml 1X PBS and vortex well. 11. Diluted Mouse anti
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Vector Laboratories公司VECTASTAIN@ ABC免疫组化试剂盒
¥3120
