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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
), H2 O2 , TdT buffer (A1), TdT enzyme (A2), biotinylated dUTP (A2), TB buffer (A1), serum albumin (BSA) (A2), PBS, Extra-avidin Peroxidase (A2), AEC solution (A1), DNAse buffer (A1). B.3. COMMENTARY B.3.1 Background
µl (50µl) of lambda phage DNA/HindIII fragments (10µg/ml).7.Non-radioactive Labeling Mix (minus DIG-dUTP):50µl (150µl) of 1mM dGTP, 1mM dATP, 1mM dCTP, 0.65mM dTTP aqueous solution.8.Deionized Water: 1.5ml of water deionized on a Milli-Q®
/330.Y mM = 37.X.1000/330.Y uM For example: B 88/77 primer - a 17-mer oligodeoxynucleotide - as supplied is 12.6 OD units/ml. We need to make a 5 uM stock solution for PCR. MW: 17 x 330 = 5610 Concentration: 12.6 OD x 37 ug/ml = 466 ug/ml
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