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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
Human/Primate IL-17F Affinity Purified Polyclonal Ab (100 ug)
- 供应商:
嵘崴达
- 库存:
需确认
- 保质期:
见产品批次
- 保存条件:
见说明书
- 规格:
100 UG
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| AF1335 | Human/Primate IL-17F Affinity Purified Polyclonal Ab (100 ug) |
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文献和实验Intracellular Cytokine Staining Protocol
TimeRestimulationIntracellular BlockAntibodyIL-1aNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinALF-161IL-1bNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinB122IL-2mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d
Phycoerythrin conjugation protocol
concentrator (Centricon 30 or 100 are fine) Apply to top of separation column. Elute with PBS. Pump column at approximately 0.3 ml/min. Order of elution is conjugate first (MW ~400,000) then free PE (MW ~240,000) then free Ab (MW ~150,000
Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
because of the known antiviral effects of this cytokine as well as its correlation to the cytotoxic capacity of T cells. However, maintenance of both IFN- and IL-2 secretion by pathogen-specific T cells has been linked to a more favorable clinical outcome in human
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