NoLimits™ 150 bp DNA Fragment

寻找KO的阳性克隆——Cruiser™酶

近年来，ZFN、TALEN、CRISPR/Cas9等基因组编辑技术可谓是层出不穷，尤其是CRISPR/Cas9，一出现便在全球范围内掀起一股热潮。同时，各大公司也开发出一系列相应的产品，其中一种至关重要的就是基因敲除阳性克隆筛选的试剂。 目前，市面上只有Genloci的Cruiser™、Transgenomic的SURVEYOR和NEB的T7EI这三种产品和测序这种方法，被用于基因敲除阳性克隆的筛选。然而，SURVEYOR是进口产品，价格贵，货期长；T7EI可以识别多种DNA结构，如十字

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

was named Red/ET recombination and the basic principles of the method are that it requires two homology regions of >42 bp in a linear fragment, double strand breaks (DSBs) in both ends, and another linear or circular plasmid in order for recombination

DNA Fragment Purification from Agarose or Acrylamide

    DNA Fragment Purification from Agarose or Acrylamide For fragments from 200 bp to 10 kb