SEROLOGICAL PIPETTE 1ML, 25/BA

Cell Cultures

with PBS. 7. Mince the embryo finely with scissors and transfer the minced tissue to a flask containing PBS. Allow the tissue pieces to settle. 8. Remove the PBS with a sterile pipette and add 25 ml of trypsin, a proteolytic enzyme. Stir the solution

Establishment of a Primary Culture

containing PBS. Allow the tissue pieces to settle.   Remove the PBS with a sterile pipette and add 25 ml of trypsin, a proteolytic enzyme. Stir the solution gently at 37° C for 15-20 minutes.   Allow the larger

原创：western protocol

1.5M Tris(PH 8.8) 100ml 18.171g (at4°C) 10% SDS 100 ml 10g (at room temperature) 10% ammonium persulfate 1ml 0.1g(replace every week store at at4°C) TEMED 8 room temprature Antibodies Primary antibody: 5A11(anti-mouse IgG) 500x-1000x Secondary