12 Strip Pre-Sterilized Cap fo

E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol

Equilibration Buffer inro the column. Incubate at room temperature for 5 minutes. Spin at maximum speed (>13,000 x g) for 1 minute. 9. Apply 750 ul of mixture from step 7 to the HiBind® Mini column II (purple) pre-inserted in a 2 ml collection tube

Cell Cultures

of an ocular grid inserted into an inverted phase contrast microscope. Cell density (cells/cm2) is then plotted on a log scale against time in culture.Exercise 12.1 - Aseptic Cell Transfers Level I Figure 12.3 Flaming a wire loop and removing cap

ICAM-1纯化 Purification of ICAM-1

ml of Elution Buffer. Elute 3ml in each prepared collection tube. Mix each tube immediately after elution and cap with parafilm to prevent evaporation during storage. Strip the column with 5 column bed volumes of Stripping Buffer. Equilibrate