DNA Ladder, High Range 1Kb-10K

Long-Range PCR with a DNA Polymerase Fusion

to mid-range targets. In this article, we present a modified protocol for using a DNA polymerase fusion to amplify genomic targets exceeding 20 kb in length. This procedure overcomes several limitations of Taq blends, which up until recently

Separation and Size Determination of DNA over a 10,200 kbp Range

7 ). Here conditions are described for the separation of DNA in the l0–,200-kbp size range by CHEF. CHEF resolution in this range exceeds that of many commonly used size standards. Production and use of a size standard giving a 10-kbp ladder in the l0–,200-kbp range

A safe method of extracting DNA from Coccidioides immitis

with 10 ul of 10 mg/ml Proteinase K for 2-3h, and then incubated again at 80 C for 10 min. The DNA is then extracted with chloroform- phenol and precipitated with isopropanol, following Lee and Taylor (In: M. A. Innis et al, eds. PCR Protocols, pp. 282-287