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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
HTScan(R) Tyrosine Kinase Buffer (4X)
- 是否单克隆：
Buffer
- 规格：
15 ml
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文献和实验is the specificity of the antibody-antigen interaction because monoclonal antibodies bind to only one epitope of the antigen. If the antigen of interest is tyrosine phosphorylated, and this tyrosine phosphorylation is under investigation, each step has to be handled
by vortexing every 10 min. Stop reaction by adding 15 µL of 4x sample buffer. Heat on 95°C block for 5 min. (Can be stored at 4°C overnight) Spin down beads and load supernatant on a 12% SDS acrylamide gel. Run off the dye front (175 volts x
Assaying Protein Kinase Activity
-transduction pathways that affect cell behavior. As an example of approaches taken to measure protein kinase activity, this chapter presents methods useful for determination of the activity of the oncogenic protein-tyrosine kinase v-Src. Included are protocols
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