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文献和实验miRNA microarrays（McManus Lab）(芯片）
d) Cut out the slice between the bands and cut apart separate samples. Use a syringe plunger to crush the gel. Place in a 15 mL falcon tube and add 1 mL of 0.3M sodium acetate for every cm in width of lane used to run sample. e) Rock overnight
Farnham Lab Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues
tissue for 2 minutes. Collect cells from medicone by inserting an 18g blunt needle and a 1 mL syringe. Put them on ice. Keep putting between 50-100 mg of tissue resuspended in 1 mL of PBS in each medicone until all the tissue is processed
v468.Chapter 8 采用 b-Catenin/TCF基本转录通讯结构对b-Catenin/TCF转录进行分析
6. Plasmids (see Note 3 )—pSL3, pSL4, pSL5, pSL9/rLuc,BAR, and fuBAR in lentiviral platform. 2.3. Lentivirus Concentration 1. 150 mL Millipore Stericup-GP PES filters (Millipore, Billerica,MA; cat. #SCGP U01 RE). 2. Beckman ultracentrifuge
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