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文献和实验Performance Comparison of the Experion Automated Electrophoresis System
IntroductionThe assessment of RNA integrity and purity is an important first step to many gene expression analysis applications. It is preferable to use high-quality, intact RNA as a starting point for applications such as RT-PCR, ribonuclease
subtypes. [J] Int Cancer, 2003, Aug. 10, 106(1): 52-59.[18]Kuo K C, McCune R A, Gehrke C W, et al. Quantitative reversed-phase high performance liquid chromatographic determination of major and modified deoxyribonucleosides in DNA [J]. Nucleic Acids Res
weights is indicative of poor RNA integrity. Tips for Optimizing Performance The area of the peaks derived from the RNA 6000 Ladder is used as a mass standard for unknowns, so accurate quantitation of your unknowns is dependent on careful handling
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