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- Chemyx
- Fusion 200
- 2025年07月03日
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1. 流速0.0001µl/min到65ml/min；
2. 最大可配置注射器体积60ml；
3. 双向推拉式
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文献和实验pipettor, 50-200 m l Pipet tips, sterile Reagent Reservoir, sterile Tupperware container Myeloma Cells One week prior to the fusion, split myeloma cells
1) In separate PCR reactions, amplify the 5' and 3' ends of the gene of interest with primers about 200 bases apart. Primer 2 should begin with 24 nts complementary to the M13 forward primer(GTC GTG ACT GGG AAA ACC CTG GCG) and primer 3 should begin
again with 30ml of IMDM. One immunized spleen has approx. 108 cells. After this wash the cells are ready for the fusion. Myeloma cell preparation: It is essential that the myelomas be free of debris, rounded and refractive under phase
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