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- 2025年07月06日
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推进式精准液体输送系统：
1.流速0.0001µl/min到102ml/min；2.最大可配置注射器体积60ml；
3.单向推进式。
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文献和实验Reagents (StemCell Technologies, Inc. # 03800) Medium A - Pre-fusion Medium and Hybridoma Expansion Medium (StemCell Technologies, Inc. - # 03801) Medium B - Fusion Medium
on the same day as the cell fusion. Cold Spring Harbor (1989) does not include the overnight incubation prior to plating. Add HAT media to the 50 ml of media in the flask (50x HAT is used at a ratio of 1 ml/100 ml of media; 500x HAT is used at a ratio
again with 30ml of IMDM. One immunized spleen has approx. 108 cells. After this wash the cells are ready for the fusion. Myeloma cell preparation: It is essential that the myelomas be free of debris, rounded and refractive under phase
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