Product Specifications
Isotype
Goat IgG
Isotype Control
Goat IgG-FITC
Specificity
Reacts with the heavy chain of human IgA
Source
Pooled antisera from goats hyperimmunized with human IgA
Cross Adsorption
Human IgG and IgM; may react with IgA from other species
Purification Method
Affinity chromatography on human IgA covalently linked to agarose
Conjugate
FITC (Fluorescein)
Buffer Formulation
Phosphate buffered saline containing < 0.1% sodium azide
Clonality
Polyclonal
Concentration
1.0 mg/mL
Volume
1.0 mL
Recommended Storage
2-8°C; Avoid exposure to light
Applications
Quality tested applications for relevant formats include -
ELISA 1-5,15
FLISA
Flow Cytometry 17,18
Other referenced applications for relevant formats include -
ELISpot 6,7
Immunohistochemistry-Frozen Sections 8,9,13
Immunohistochemistry-Paraffin Sections 10,11
Immunocytochemistry 12
Western Blot 14,15
Immunoprecipitation 16
Multiplex 1,19
Purification 21,22
Depletion 20
RRID Number
AB_2795702
SouthernBiotech(Southern Biotechnology Associates, Inc)是由美国阿拉巴马大学伯明翰分校(UAB)Dr. Max Cooper细胞免疫学实验室中的科学家们于1982年创立,成立30年来一直致力于生产高质量、高亲和纯化的二抗、标记二抗以及其它免疫试剂,包括多种类型的多克隆和单克隆抗体、胶原蛋白和其它细胞外基质蛋白。
1. Dasso J, Lee J, Bach H, Mage RG. A comparison of ELISA and flow microsphere-based assays for quantification of immunoglobulins. J Immunol Methods. 2002;263:23-33. (ELISA, Multiplex)
2. Wang J, Anders RA, Wu Q, Peng D, Cho JH, Sun Y, et al. Dysregulated LIGHT expression on T cells mediates intestinal inflammation and contributes to IgA nephropathy. J Clin Invest. 2004;113:826-35. (ELISA)
3. Yang M, Li F, Xie X, Wang S, Fan J. CagA, a major virulence factor of Helicobacter pylori, promotes the production and underglycosylation of IgA1 in DAKIKI cells. Biochem Biophys Res Commun. 2014;444:276-81. (ELISA)