DAPI Fluoromount-G® is a water-soluble, 4',6-diamidino-2-phenylindole (DAPI) containing compound for slides mounted after a staining procedure having an aqueous final step. It is suitable for microscopy applications utilizing frozen and paraffin sections, whole mounts, and cells requiring nuclear visualization.
图4.HCC827细胞先用抗E-钙粘蛋白抗体染色,随后用二抗染色,最后用DAPI Fluoromount-G®(货号0100-20)封片。图片来自Narita M, Shimura E, Nagasawa A , Aiuchi T , Suda Y ,Hamada Y et al . Chronic treatment of non-small-cell lung cancer cells with gefitinib leads to an epigenetic loss of epithelial properties associated with reductions in microRNA-155 and -200c. PLOS One.2017; 12 (2): e0172115.
图5.在无血清培养基中生长的人中性粒细胞用抗中性粒细胞弹性蛋白酶抗体染色,然后用AF555偶联的二抗染色,并用DAPI Fluoromount-G®(货号0100-20)封片。图片来自Kamoshida G, Kikuchi-Ueda T, Nishida S, Tansho-Nagakawa S , Kikuchi H, Ubagai T , et al . Spontaneous formation of neutrophil extracellular traps in serum-free culture conditions. FEBS Open Bio. 2017; 7: 877-86.
SouthernBiotech(Southern Biotechnology Associates, Inc)是由美国阿拉巴马大学伯明翰分校(UAB)Dr. Max Cooper细胞免疫学实验室中的科学家们于1982年创立,成立30年来一直致力于生产高质量、高亲和纯化的二抗、标记二抗以及其它免疫试剂,包括多种类型的多克隆和单克隆抗体、胶原蛋白和其它细胞外基质蛋白。