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- 罗氏 -11277049001
- 2026年03月30日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
见产品外包装
- 英文名：
Deoxynucleoside Triphosphate Set
- 库存：
需确认
- 供应商：
嵘崴达
- CAS号：
见产品外包装
- 保存条件：
见产品外包装
- 规格：
4 x 100 μL
Deoxynucleoside Triphosphate Set
Lithium salt
别名: datp, dctp, dgtp, dttp, set of datp, dctp, dgtp, dttp
| packaging | pkg of 4 × 100 μL (11277049001 [4x10 μmol]) |
| pkg of 40 × 100 μL (11922505001 [40x100mM]) |
General description
Deoxynucleoside Triphosphate Set consists of four separate vials of dATP, dCTP, dGTP, and dTTP. Each vial contains a 100 mM clear, colorless solution of the lithium salt (pH 7).
Application
• Absorbance determination: A250/A260, A280/A260, A290/A260
• DNase activity negative, according to the current Quality Control procedures
• Suitability for PCR
Use the Deoxynucleoside Triphosphate Set, lithium salt, for PCR, RT-PCR, DNA sequencing/cycle sequencing, labeling, Reverse Transcription and all types of amplification reactions or primer-extension reactions.
包装
1 set containing 4 components
Preparation Note
Activator: Mg2+
Working solution: If required, the solutions can be diluted with distilled autoclaved water.
| 11277049001 | Deoxynucleoside triphosphate S脱氧核苷三磷酸 | 4 x 10 µmol (4 x 100 µl) |
| 11418432001 | Taq DNA Polymerase,5units/Ál(4 Taq DNA聚合酶 | 1,000 U (4 x 250 U) |
| 11647687001 | Taq DNA Polymerase,1unit/Ál (4 Taq DNA聚合酶 | 1,000 U (4 x 250 U) |
| 10380385001 | Restr.-Endonucl. Hpa I, 100 u | 100 U (3 - 10 U/µl) |
| 11596594001 | Taq DNA Polym., 5 units/Ál (10 Taq DNA聚合酶 | 2,500 U (10 x 250 U) |
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文献和实验Assays for Hepatitis B Virus DNA-and RNA-Dependent DNA Polymerase Activities
of nucleoside analogs. In vitro assays for inhibition of HBV Pol functions by deoxynucleoside triphosphate (dNTP) analogs are useful indicators but, because of restrictions imposed by hepatocyte enzymology, provide no guarantee of potential anti-HBV activity
Direct Sequencing of PCR Products Using Chemiluminescent Detection
published (1 ). Direct sequencing of PCR products is generally more difficult than sequencing single-stranded DNA (2 ) owing to the presence of primers and deoxynucleoside triphosphates (dNTPs) carried over from the PCR into the sequencing phase
Biochemical Assays for the Characterization of DNA Helicases
Helicases are ubiquitous enzymes that disrupt complementary strands of duplex nucleic acid in a reaction dependent on nucleoside-5′-triphosphate hydrolysis. Helicases are implicated in the metabolism of DNA structures that are generated
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