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- 11449460001
- 2025年10月21日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
见产品外包装
- 英文名：
DNA Molecular Weight Marker IX
- 库存：
需确认
- 供应商：
嵘崴达
- CAS号：
见产品外包装
- 保存条件：
−20°C
- 规格：
50 μG
DNA Molecular Weight Marker IX
Application
DNA Molecular Weight Marker IX is especially designed for the analysis of mid-size PCR products in agarose gels. It simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:
• Restriction digests
• PCR
• RT-PCR
Sequence
The mixture contains eleven DNA fragments with the following base pair lengths (1 base pair = 660 Daltons): 72, 118, 194, 234, 271, 281, 310, 603, 872, 1078, and 1353.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
Unit Definition
50 μg = 1A260 unit
Preparation Note
Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.
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文献和实验Molecular Weight Marker l Hin dIII Digest Marker Solution Digest 20 µg l DNA (40 µl DNA if at 0.5 µg/µl) Add 50 µl 10X Tracking Dye Bring volume of the digested DNA up to 500 µl
The Isolation of High Molecular Weight Eukaryotic DNA
The isolation of high molecular weight eukaryotic DNA in good yield is an important prerequisite for the analysis of specific sequences by Southern blotting (Chapter 9 ), or for molecular cloning in phage or cosmid vectors (Chapter 49 ).
High Molecular Weight DNA Extraction from Arabidopsis
Large DNA molecules (>l00 kb) are extremely sensitive to mechanical shearing m aqueous solution. Therefore, classical DNA extractron procedures from living tissues generally do not allow recovery of DNA fragments larger than 50‐100 kb m size
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