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- 罗氏 11558706910
- 2025年11月14日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Digoxigenin-11-dUTP, alkali-stable
- 保存条件：
−20°C
- 供应商：
嵘崴达
- 库存：
需确认
- 保质期：
见产品外包装
- 规格：
125 μL
Digoxigenin-11-dUTP, alkali-stable
| packaging | pkg of 125 μL (11558706910 [1 mM]) |
| pkg of 5 × 125 μL (11570013910 [5x1 mM]) | |
| pkg of 25 μL (11093088910 [1 mM]) |
Application
Digoxigenin-11-dUTP, alkali-stable has been used to label probes for
fluorescent in situ hHybridization (FISH) and polymerase chain reaction (PCR)
Note: When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will remain attached. It is therefore ideal for use in DIG-labeled DNA that needs to survive exposure to alkaline treatment. However, if you intend to make a DIG-labeled probe that can be removed by alkali denaturation (e.g ., during stripping and reprobing of blots), do not use this alkali-stable preparation. Instead, use the alkali-labile form of DIG-11-dUTP.
Biochem/physiol Actions
Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) replaces deoxythymidine triphosphate (dTTP) in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% DIG-11-dUTP and 65% dTTP. DIG-11-dUTP can also replace dTTP in labeling during polymerase chain reaction (PCR) (e.g., with Taq DNA Polymerase), 3′-tailing (with terminal transferase) and cDNA synthesis (e.g., with transcriptor or M-MuLV reverse transcriptase). It is ideal as a substrate for DNA polymerase, Taq DNA polymerase, terminal transferase and reverse transcriptase.
Features and Benefits
耐碱性：在+ 15至+ 25℃条件下，该制剂在0.1-0.5M NaOH 存在时稳定。
Quality
典型分析：至少 85％ DIG-11-dUTP（HPLC，面积％）。
通过 RPL 测试的功能。
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文献和实验Random Prime Labeling of DNA Probes with Fluorescein-11-dUTP
The selection of an appropriate labeling reagent for a particular experiment, for the most part, depends on the sensitivity and resolution required. For maximum sensitivity in filter hybridizations radioactive labels, such as phosphorous 32
3′-End Labeling of Oligonucleotides with Fluorescein-11-dUTP and Enhanced Chemiluminescent Detection
In this reaction, the enzyme terminal transferase is used to introduce a short tail of fluorescein nucleotides to the 3′-end of an oligonucleotide (1 ) followed by horseradish peroxidase-catalyzed enhanced chemiluminescent detection
分布在寒温带到热带的亚湿润乃至干燥地区的一种土壤。是指土壤反应为pH值 8.5以上的强碱性，在胶体上吸附的代换性钠占土壤阳离子代换量的 15%以上，并含有可使大部分作物生长达到受害程度的钠的土壤。这种土壤可分为：排水不良，干燥期碱性盐类上升，在地表上形成白色盐皮的盐土（英 solo-nchak；俄солончак）；排水良好，碳酸钠溶解腐植质向下层浸透，使土层呈现黑色的碱土（英 solonetz；俄солонец）。 在这种土壤上，只有耐碱性与耐高浓度盐类的盐生植物和旱生植物才能稀疏
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