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- roche
- 11603558001
- 2026年03月24日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
见产品外包装
- 英文名：
DIG Easy-Hyb
- 库存：
需确认
- 供应商：
嵘崴达
- CAS号：
见产品外包装
- 保存条件：
2-8°C
- 规格：
500 mL
DIG Easy Hyb™
suitable for hybridization, solution
别名:
DIG system
一般描述
DIG Easy Hyb is a nontoxic, ready-to-use hybridization buffer. DIG Easy Hyb does not contain formamide, but facilitates lowering of hybridization temperature comparable to buffers that contain 50% formamide.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
应用
DIG Easy Hyb can be used in all membrane hybridization applications using nonradioactive (e.g., digoxigenin-labeled) nucleic acid probes. DIG Easy Hyb is ready-made, and can be used without any additions. It is specifically designed for use with DIG kits.
质量
Absence of contaminants: DNase- and RNase-free.
| 11603558001 | DIG Easy-Hyb | 500 ml | |
| 11721925001 | DNA MW-Marker XIII | 50 µg (1 A260 unit) | |
| 11855638001 | DNA MW-Marker XVI | 50 µg (200 µl) | |
| 11855646001 | DNA MW-Marker XVII | 50 µg (200 µl) | |
| 11666649001 | Hybridization Bags | 50 bags | |
| 10901393001 | Glycogen , MB Grade 糖原 | 20 mg (1 ml) | |
| 11140922001 | CTP, lithium salt | 40 µmol (400 µl) | |
| 11140949001 | UTP, lithium salt | 40 µmol (400 µl) | |
| 11140957001 | GTP, Solution | 40 µmol (400 µl) | |
| 11140965001 | ATP, lithium salt | 40 µmol (400 µl) |
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文献和实验) and Whatman paper in the 20X SSC that remains. Set up transfer without a SSC resevoir, let stand overnight. Hybridization Prehyb: Incubate blot with 30mls of DIG Easy Hyb (Roche) for at least 1 hr at 42o C. Prehyb solution can be reused
Pre-hyb/hyb for Radioactive Probes
Pre-hyb/hyb for Radioactive Probes Stuff you need: 50X Denhardt's reagent: 1% Ficoll 1% Polyvinylpyrrolidone 1% Bovine Serum Albumin Prehyb/hyb solution: 5X SSC 50% formamide 100 µg/ml SS DNA (heat denatured at 95 deg C) 5X
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