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文献和实验.M. et al. (1989) Nucl. Acids Res., 17, 3469�3478. (10) Raleigh, E.A., Trimarchi, R., and Revel, H. (1989) Genetics, 122, 279�296. (11) Grant, G.N. et al (1990) Proc. Natl. Acad. Sci. USA , 87, 4645�4649. (12) Krüger, T. et al. (1992) Gene, 114, 1�
Transformation of E. coli by Electroporation
and then pressing the UP voltage control button until the voltage reading is 5 to 10 volts higher than the desired discharge voltage. For E. coli , the standard conditions are 2.4 kv, which means setting the Pulse Control unit to 405 volts (400 volts is the desired
Expression and Purification of CRABPs from E. coli
Nuclear-retinoid receptors provide a mechanism of retinoid action, but most likely where expressed, the cytosolic cellular retinoic acid-binding proteins (CRABPs) also affect the ability of retinoids to initiate biological signals
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