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Neobioscience
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文献和实验Activation of Nuclear Factor-B (NF-B) in Mononuclear Cells (MNC)
The transcription factor NF-κB was first identified as a regulator of the expression of the kappa light-chain gene in murine B lymphocytes (1 ). It has a central role in the immunological processes and regulates a wide range of genes
Activation of Nuclear Factor-B
was determined. We also demonstrate the specificity of the NF-κB binding activity using a competition and supershift assay with specific antibodies that recognize NF-κB subunits p50 and p65. Lipopolysaccharide caused a rapid and significant increase in NF-κB
Factor Assay kit试剂盒测定胞核提取物中p65蛋白与DNA靶序列结合能力。 结果表明:(1)氯化镧对TNF-α诱导的IκBa磷酸化和降解的抑制作用:TNF-α能够诱导Hela细胞中IκBa的磷酸化和降解。随着氯化镧处理的浓度不断升高,IκBa表达水平逐步升高,而磷酸化水平不断降低。表明氯化镧能够抑制TNF-α诱导的IκBa的磷酸化和表达水平的降低,且该效应随着随着氯化镧浓度的升高而逐步增强。(2)氯化镧对TNF-ct诱导的NF-κB活化的抑制作用:25―100μmol/L氯
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