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文献和实验TUNEL Assay for Apoptotic Cells
, apoptosis is genetically driven and is characterized by chromatin condensation, nuclear shrinkage and eventual loss of nuclear membrane, membrane blebbing that produces apoptotic bodies containing cellular organelles and chromatin, DNA degradation
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
The immunocytochemical procedure described represents an original modification of the method for cell death detection by ELISA. Utilizing this new modified assay we are able to show the presence of apoptotic cells or cells involved in apoptosis in the thymus
1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
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