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文献和实验Assessment of 5-Lipoxygenase Activity and Cellular Distribution
5-Lipoxygenase (5-LO) catalyzes the conversion of arachidonic acid to leukotriene mediators of inflammation (1 ,2 ). Leukotriene biosynthesis in intact cells involves redistribution of 5-LO from a soluble cellular compartment
Recombinant Baculovirus Isolation
Although there are several different methods available of making recombinant baculovirus expression vectors (reviewed in Chapter 3), all require a stage in which insect cells are transfected with either the virus genome alone (Bac-to-Bac�
or ladder in kDa), and also give you information on protein expression (with comparison to a control such as untreated sample or another cell type or tissue). Summary: Western Blot Gives You Information on the: Size of your Protein
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