Pwo SuperYield DNA Polymerase

Pwo SuperYield DNA Polymerase

suitable for PCR, Difficult Templates/Specialty Enzymes PCR, hotstart: no, dNTPs included: no

别名:
polymerase, dna, pwo superyield

一般描述

Pwo SuperYield DNA Polymerase combines the recombinant enzyme Pwo DNA Polymerase with an optimized buffer system. This buffer system enhances the enzymatic properties of the polymerase, resulting in higher yields of the amplification reaction without changing the fidelity of DNA synthesis. This enzyme delivers excellent results due to its enzyme design and optimized buffer system. Amplify fragments up to 3 kb - even longer amplicons are possible from simple templates.
Pwo SuperYield DNA Polymerase exhibits increased thermal stability with a half life of greater than 2 hours at +100 °C compared to Taq DNA Polymerase with a half life of less than 5 min at this temperature.
Pwo SuperYield DNA Polymerase, originally isolated from Pyrococcus woesei, is a processive 5′ →3′ DNA polymerase with 3′ →5′ exonuclease proofreading activity; 5′ →3′ exonuclease activity is not detectable.
The enzyme accepts modified nucleotides for efficient labeling of nucleic acids by PCR.
PCR products are blunt-ended directly useable for blunt-end ligation.
Using the magnesium-containing reaction buffer supplied, the final MgCl₂ concentration is 1.5mM.

特异性

Star activity: In cases where star activity is observed and/or the activity of the enzyme in the PCR mix is low, first purify the amplification product prior to the restriction enzyme digest using High Pure PCR Product Purification Kit.

应用

• Pwo Super Yield DNA Polymerase combines the recombinant enzyme Pwo DNA Polymerase with a newly optimized buffer system. Pwo SuperYield DNA Polymerase is used for the amplification of DNA with the intent to sequence the amplification product or to clone the product (e.g., for the expression of the gene product). The high fidelity of this enzyme makes it particularly suitable for: High fidelity PCR
• Site-directed mutagenesis
• Cloning
• Gene expression
• Study of allelic polymorphism in individual RNA transcripts
• Characterization of rare mutations in tissue
• Characterization of the allelic stage of single cells or single DNA molecules

特点和优势

Pwo SuperYield DNA Polymerase yields more high fidelity PCR product. The optimized buffer delivers superior results. Amplify fragments up to 3kb. A GC-RICH Solution is included for difficult templates.

• Higher yield and 18-fold higher fidelity

compared to Taq DNA Polymerase.

• High performance with difficult templates.

The GC-RICH Solution is for GC-rich PCR.

• Reduce working steps in cloning.

Perform digests directly in Pwo SuperYield PCR mix.

包装

1 kit containing 3 components