Bovine Serum Albumin Fraction V is suitable for standard applications such as: • Stabilizer to prevent proteolysis and denaturation of enzymes and other proteins, especially when the proteins are present in low concentrations. • Buffer component in immunochemistry, biochemistry, cell biology, or molecular biology • Reducing agent • Protein blocker for saturation of protein binding sites in ELISAs, Southern blots, and western blots • Media supplement for media that require addition of protein • Carrier protein • Standard protein in gel electrophoresis and protein determination
Quality
Purity: ≤95% protein, ≤5% H2O; electrophoretically homogeneous Contaminants: <0.5% Na (flame photometric), <0.006% K (flame photometric), <0.0003% Li (flame photometric), <0.02% Ca (o-cresolphthalein), <0.003% Mg (xylidyl blue), <0.003% heavy metals (as Pb), <0.001% Fe (bathophenanthroline), <0.002% Pi, <0.05% glucose (enzymatic), <0.005% glycerol (enzymatic), <0.025% L-lactate (enzymatic)
Preparation Note
Storage conditions (working solution): In solution, albumin is stable for about three months at -15 to -25 °C.