The FastStart™ SYBR® Green Master has been used in qPCR and two-step qRT-PC in the SYBR® Green I detection format. It is also used: • in quantitative polymerase chain reaction (qPCR) for adeno-associated virus (AAV) titre quantification • in qPCR to determine the expression level of different Col6a1-3 genes relative to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) • in quantitative real-time polymerase chain reaction (qRT-PCR) of G protein-coupled estrogen receptor-1 (GPER) mRNA • in qRT-PCR for gene expression studies
Use the FastStart™ SYBR® Green Master with any real-time qPCR instrument other than the LightCycler® Instruments.