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文献和实验Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
Purification of SUMO Conjugating Enzymes and Kinetic Analysis of Substrate Conjugation
SUMO conjugation to protein substrates requires the concerted action of a dedicated E2 ubiquitin conjugation enzyme (Ubc9) and associated E3 ligases. Although Ubc9 can directly recognize and modify substrate lysine residues that occur
Stability and Ubiquitination of the Tumor Suppressor Protein p53
-dependent manner through its covalent thio-ester linkage to the E1 ubiquitin-activating enzyme. The activated ubiquitin is then transferred to the E2 enzyme, also known as a ubiquitin conjugating enzyme (UBC), again in the form of a high-energy thio-ester
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