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- Enzo Life Sciences
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- BML-PW1220-0025
- 2025年07月13日
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文献和实验Studies of the Ubiquitin Proteasome System
6.6 ), autoradiography and phospher imaging (unit 6.3 ), or immunoblotting (unit 6.2 ) Alternate Protocol 1: Binding of Ubiquitin Substrates to E3s In vitro–translated 35 S‐labeled substrate protein
A Simple Solid-Phase Competition Assay with Labeled Antigen
is time consuming and tedious, and the instability of radiolabels represents a significant drawback. In addition, radioactive hazards have to be taken into account. Recently, nonisotopic tracers, such as biotin ( 3 , 4 ) and fluorescein isothiocyanate
A Solid-Phase Mutual Inhibition Assay with Labeled Antigen
was incubated with a given MAb immobilized on wells of 96-well plates in the presence of increasing amounts of soluble competitor MAbs. The biotinylated CEA bound to the immobilized antibody was then reacted with avidin–peroxidase conjugate and the activity
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