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- Enzo Life Sciences
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- 2025年07月14日
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文献和实验Coimmunoprecipitation Assay for the Detection of Kinase-Substrate Interactions
Coimmunoprecipitation is a powerful tool to study protein-protein interactions and can be used to test for the physical association between a known protein kinase and its substrate. In this chapter, the author describes a protocol
Monitoring Protein Kinase A Activities Using Expressed Substrate in Live Cells
Protein kinase A (PKA) activity is regulated by intracellular cyclic adenosine mono-phosphate. Conventional protein kinase assays after cell lysis are hence not suitable for analyzing PKA activities. In this chapter, we describe a new method
Western Blotting with Biotinylated Antibodies
7.5, 100mM NaCl, 0.1% Tween 20). 2.Incubate the blot for 30 minutes at 37℃, 1 hour at room temperature, or overnight at 4℃. Incubation with Biotinylated Primary Antibody Primary antibody 1.Dilute the antibody in the corresponding blocking buffer
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