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文献和实验Purification of recombinant sBRF M166L
, then 0.75M, and then 0M Urea. Spin renatured protein in the SS34 rotor for 10 min at 10K rpm. If a large pellet is detected, analyze the samples by SDS PAGE before proceeding with the purification to confirm that the renatured BRF is soluble. BRF
Purification of recombinant sBRF M166L
PAGE before proceeding with the purification to confirm that the renatured BRF is soluble. -------------------------------------------------------------------------------- BRF Renaturation Buffer (1.5 liters) 10% glycerol 150 ml glycerol 20 mM
Many cytokines alter the half-lives and stabilities of mRNAs for other cytokines (1 ) and enzymes that may be related to disease progression. For example, IL-1β stabilizes the mRNA for human interstitial collagenase-1 in normal fibroblasts
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