High potency inhibitor of smooth muscle contractions
Applications in nephrology, stem cell studies, cardiovascular, cancer, and neurology
Highly cited
Y-27632 is a highly potent, cell permeable, selective and ATP competitive inhibitor of ROCK1 and ROCK2 (IC50=800nM). It acts as a potent inhibitor of agonist-induced Ca2+sensitization of myosin phosphorylation and smooth muscle contractions, blocking cell spreading and suppresses RhoA-induced formation of stress fibers in hepatic stellate cells. Y-27632 significantly reduces the increase of inflammatory cytokines after reperfusion, preventing the development of acute renal failure. There are numerous application for this compound including: cardioprotective effects, suppression of tumor cell invasion, inhibition of superoxide production, mimicking effect of β-agonists on human cells, and an antinociceptive effect.
There has been a growing interest in Y-27632 for use in stem cell self-renewal and reprogramming. It is known to increase the survival rate of human embryonic stem cells undergoing cryopreservation.
Product Details
Alternative Name: (R)-(+)-trans-N-(4-Pyridyl)-4-(1-aminoethyl)-cyclohexanecarboxamide . 2HCl
Formula: C14H21N3O . 2HCl
MW: 247.3 . 73.0
CAS: 129830-38-2
Purity: ≥98% (HPLC (UV))
Purity Detail: Enantiomeric excess ≥96%
Identity: Determined by MS and NMR.
Appearance: White to pale yellow powder.
Solubility: Soluble in DMSO (25mg/ml), methanol (25mg/ml), acetonitrile (<1mg/ml), dioxan (<1mg/ml), water (very soluble).
Shipping: Ambient
Long Term Storage: -20°C
Use/Stability: Stock solutions are stable for up to 1 month when stored at -20°C.
Handling: Protect from light. Packaged under inert gas. After reconstitution, prepare aliquots and store at -20°C.