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文献和实验Coupling Peptides To Resin For Affinity Purification
Coupling Peptides To Resin For Affinity Purification Claire Walczak January 1996 We use Affigel-10, converting its functional group first to amino and then to iodoacetyl. You can also buy amino resin (it's
Coupling Peptides To Resin For Affinity Purification
We use Affigel-10, converting its functional group first to amino and then to iodoacetyl. You can also buy amino resin (it's more expensive) and start at step 6. All washes are performed on a glass-fritted filter
A Method for Assaying Deubiquitinating Enzymes
residue in the PESTc portion of the fusion protein was almost exclusively radioiodinated under a mild labeling condition, such as using IODO-BEADS, the enzymes could be assayed directly by simple measurement of the radioactivity released into acid soluble
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